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Naph-EA-mal

CAS No. : 210292-65-2

MCE 站：Naph-EA-mal

产品活性：Naph-EA-mal (Thiol-green 1) 是一种快速检测和超快 turn-on 型硫醇 (thiol) 荧光探针，可用于蛋白标记和生物成像。Naph-EA-mal (Thiol-green 1) 可用于检测活细胞中的硫醇，标记蛋白质硫醇，定量细胞裂解液中总硫醇的浓度，并确定固定细胞中可逆的蛋白质硫醇氧化。Ex: 488 nM; Em: 540 nM。

研究领域：Others

作用靶点：Others

In Vitro: Imaging thiols in living cells
Hep G2 cells incubated with Naph-EA-mal (Thiol-green 1) (1 µM;5 mins), ａnd strong green fluorescence can be observed. When cells are preincubated the cells to block the intracellular thiols with NEM (a thiol block reagent; 100 µM), a weaker fluorescence is observed in cells.
Monitoring thiol-disulfide levels in fixed cells
Due to the majority of cellular thiols are present in the reduced form, Naph-EA-mal (Thiol-green 1) (10 µM) induces strong fluorescence in Hep G2 cells. After diamide treatment, fluorescent signal sharp dimed is observed.
The Hep G2 cells, oxidized by diamide (200 µM; 30 min), are fixed by 70% EtOH ａnd the remaining free thiols are blocked by NEM (100 µM). then The cells are further treated with TCEP (5 mM) followed by addition of the Naph-EA-mal (Thiol-green 1) (10 µM). The green fluorescent signal is detected again

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