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BODIPY-Cholesterol

CAS No. : 878557-19-8

MCE 站：BODIPY-Cholesterol

产品活性：BODIPY-cholesterol 是一种带有荧光 BODIPY 基团的亲脂性和细胞通透性胆固醇类似物。BODIPY-cholesterol 可用于监测细胞内固醇摄取和细胞器间固醇通量。(激发/发射波长: 480/508 nm)。

研究领域：Others

作用靶点：Others

In Vitro: BODIPY-cholesterol assay
To prepare a stock solution of BODIPY-cholesterol for this assay, the analogue is reconstituted in DMSO to a stock concentration of 1-10?μM.
Cells diluted with PBS (or other buffers) are washed (300×g, 10?min) to remove excess diluent and the loose pellet resuspended with vehicle media to a proper concentration.
Cell suspensions are labeled with BODIPY-cholesterol (final concentration of 0.5-5??M), mixed thoroughly and incubated for 10?-20 mins at 37?°C.
The cells are diluted with each of the capacitation media conditions as outlined in Incubation media to a final concentration and incubated for 2?h prior to flow cytometric assessment.

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