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Fluo-4 AM

CAS No. : 273221-67-3

MCE 站：Fluo-4 AM

产品活性：Fluo-4 AM 是一种常用的检测细胞内 Ca²⁺ 浓度的探针。

研究领域：Others

作用靶点：Others

In Vitro: Fluo-4 AM is a fluorescent dye (λ_ex=494 nm, λ_em=516 nm). Preloaded with Fuo-4 AM, a very bright fluorescence image is observed. In a parallel experiment with fluo-3 AM-loaded cells, the resulting fluorescence image, although clearly discernable in this case, is less bright.Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1.Count the cells and take 106 cells from each sample (control and experiment/s).
2.Collect the cells (5 min, 3000×g, 4 °C) and wash once in PBS.
3.Resuspend the cells in 0.5-ml PBS and add 0.5 μl of Fluo-4-AM (1 mM stock) to a final concentration of μM. Incubate at 37 °C for 1 h.
4.Wash the cells three times (2 min, 3000×g) with PBS and finally resuspend in 1-ml PBS. Separate into 2 flow cytometry tubes—0.5 ml in each.
5.Evaluate the staining by flow cytometry and analyze the data by a software such as CellQuest software.

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