MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务。

TPC2-A1-P

CAS No. :

MCE 站：TPC2-A1-P

产品活性：TPC2-A1-P?是一种强大的膜渗透性的双孔通道 2 (TPC2) 激动剂 (EC₅₀=10.5 μM)，它通过模仿 PI(3,5)P2 的生理作用发挥作用。与 TPC2-A1-N (HY-131614) 相比，TPC2-A1-P 具有更高的诱导 Na⁺ 活化的能力，可用于检测完整细胞中 TPC2 通道的不同功能。

研究领域：Membrane Transporter/Ion Channel

作用靶点：Sodium Channel

In Vitro: Two-pore channels (TPC1-3) are ancient members of the voltage-gated ion channel superfamily. TPCs are expressed throughout the endo-lysosomal system and regulates the trafficking of various cargoes.
TPC2 can mediate different physiological and possibly pathophysiological effects depending on how it is activated.?The?ion selectivity of TPC2 is not fixed but rather agonist-dependent. TPC2 is a unique example of an ion channel that conducts different ions in response to different activating ligands.
TPC2-A1-P (10 μM) reproducibly evokes Ca²⁺?signals, and TPC2-A1-P response reachs its plateau slower than TPC2-A1-N (HY-131614). The EC₅₀?in full concentration-effect relationships for the plateau response is 10.5 μM for TPC2-A1-P in a cell line stably expressing TPC2^L11A/L12A.
TPC2-A1-P (10-30 μM) induces Ca²⁺?signals in Hela cells expressing TPC2 in the presence but not absence of extracellular Ca²⁺. However, the responses are smaller and delayed compared to TPC2-A1-N (HY-131614), consistent with the results obtained in cells stably expressing TPC2^L11A/L12A. TPC2-A1-P fails to induce Ca²⁺?signals in cells expressing ‘pore-dead’ TPC2^{L11A/L12A/L265P} and also fails to evoke Ca²⁺?signals in cells expressing human TRPML1 re-routed to the plasma membrane (TRPML1^ΔNC).
In endo-lysosomal patch-clamp experiments, TPC2-A1-P (10 μM) evokes currents in endo-lysosomes isolated from cells expressing TPC2 and TPC2^M484L, the currents evoked by TPC2-A1-P are significantly larger than those evoked by TPC2-A1-N (HY-131614) in both wild-type and gain-of-function variant,and exhibits an EC₅₀?value of 0.6 μM for TPC2-A1-P.

相关产品：Bioactive Compound Library Plus  |  Membrane Transporter/Ion Channel Compound Library  |  EIPA  |  Amiloride hydrochloride  |  Monensin sodium salt  |  Carbamazepine  |  5-(N,N-Hexamethylene)-amiloride  |  Riluzole  |  20(S)-Ginsenoside Rg3  |  Lidocaine  |  Amitriptyline hydrochloride  |  Cariporide  |  Lamotrigine  |  Ranolazine dihydrochloride  |  Flecainide acetate  |  Phenytoin  |  Zonisamide  |  Veratridine  |  Oxcarbazepine  |  A-803467  |  Triamterene  |  Flunarizine dihydrochloride  |  Vinpocetine  |  QX-314 chloride  |  Dronedarone  |  Propafenone hydrochloride  |  Mexiletine hydrochloride  |  Topiramate

品牌介绍:
•   MCE (MedChemExpress) 拥有数百种全球独家化合物，我们致力于为全球科研客户提供*新*全的高品质小分子活性化合物；
•   10,000 多种高选择性抑制剂、激动剂涉及各热门信号通路及疾病领域；
•   设有专业的实验中心和严格的质控、验证体系；
•   提供 LC/MS、NMR、HPLC、手性分析、元素分析等各项质检报告，确保产品的高纯度、高品质；
•   产品的生物活性多经各国客户实验验证；
•   Nature, Cell, Science 等多种期刊及制药收录了MCE客户的科研成果；
•   专业团队跟踪*新的制药及生命科学研究进展，为您提供全球*新的活性化合物；
•   与世界各大制药公司及知名科研机构建立了长期的合作。