RT Master Mix for qPCR II (gDNA digester plus)

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RT Master Mix for qPCR II (gDNA digester plus) 反转录即用型预混液，采用升级款反转录酶，反应更快速，适用于高 GC 含量、复杂二级结构、少量 RNA 模板等。试剂盒配有gDNA digester可去除RNA模板中的基因组DNA污染。

Description & Advantages:

MCE RT Master Mix is a convenient, ready-to-use formulation for reverse transcription. This kit contains 5x gDNA digester Mix which can eliminate gDNA contaminations in RNA samples. 4× Super RT Mix contains all the reagents necessary for first-strand cDNA synthesis. In addition, the increased heat resistance of the advanced reverse transcriptase allows reverse transcription to be performed at a higher temperature, increasing the efficiency ａnd specificity of cDNA synthesis.

Upon completion of the first-strand cDNA synthesis, the cDNA product can be directly applied as a template in a standard PCR ａnd qPCR. MCE SYBR Green qPCR Master Mix (HY-K0501) is highly recommended for detection of the expression levels of interested genes.

Features:

1. Synthesize cDNA rapidly ａnd specifically.

2. Super-fast gDNA removal in 2 minutes.

3. Optimized buffer enhances sensitive ａnd reliable cDNA synthesis.

4. Super-efficient reaction even with low template amounts (total RNA: 50 pg -5 μg in a 20 μL reaction).

5. The kit provides both Oligo dT Primer ａnd Random Primer.