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Nile Red

CAS No. : 7385-67-3

MCE 站：Nile Red

产品活性：Nile Red (Nile Blue A oxazone) 是一种针对细胞内脂质小滴和中性脂质的选择性疏水性荧光染料。Nile Red 在所有有机溶剂中均具有强烈的荧光性，其荧光颜色范围从金黄色到深红色。

研究领域：Others

作用靶点：Others

In Vitro: Nile red-stained, lipid droplet-filled macrophages exhibit greater fluorescence intensity than does Nile red-stained control macrophages, ａnd the two cell populations could be differentiated ａnd analyzed by flow cytofluorometry. Better selectivity for cytoplasmic lipid droplets is obtained when the cells are viewed for yellow-gold fluorescence (excitation, 450-500 nm; emission, greater than 528 nm) rather than red fluorescence (excitation, 515-560 nm; emission, greater than 590 nm).
Nile red is strongly fluorescent, but only in the presence of a hydrophobic environment. Nile red is very soluble in the lipids it is intended to show, ａnd it does not interact with any tissue constituent except by solution.
Spectral ａnd physicochemical properties of the lipophilic dye Nile red induce a yellow-gold-spectral shift in its excitation-emission peak, allowing it to fluoresce in the green emission spectrum only when in a lipid-rich environment, but not in more polar environments.

In Vivo: When Nile red-stained Caenorhabditis elegans is viewed for green fluorescence, discrete lipid bodies can be observed throughout the intestine ａnd other tissues either in clusters or evenly dispersed, depending on the animal＇s genotype or experimental treatment.

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