MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务。

NIR-H2O2

CAS No. :

MCE 站：NIR-H2O2

产品活性：NIR-H2O2 是一种可透过细胞的 NIR 荧光启动传感器。NIR-H2O2 在 NIR 区域内同时具有吸收和发射作用。当在 NIR 区域激发时，NIR-H2O2 对 H₂O₂ 产生较大的启动 NIR 荧光信号。NIR-H2O2 能够对活细胞和小鼠中内源性产生的 H₂O₂ 进行成像。

研究领域：Others

作用靶点：Others

In Vitro: NIR-H2O2 is highly selective to H₂O₂ over other typical ROS ａnd biorelevant species.
HeLa cells incubated with NIR-H2O2 (5 μM) for 30 min at 37 °C provide almost no fluorescence. However, when the living HeLa cells loaded with NIR-H2O2 are further treated with H₂O₂, they give strong fluorescence. NIR-H₂O₂ is cell membrane permeable ａnd responsive to H₂O₂ in the living cells. When stimulated by phorbol myristate acetate (PMA), macrophage cells may produce endogenous H₂O₂. The living RAW264.7 macrophage cells loaded with only the NIR sensor NIR-H2O2 (1 μM) display almost no fluorescence. However, the macrophage cells coincubated with PMA (3.0 μg/mL) ａnd the sensor NIR-H2O2 (1 μM) exhibit a dramatic enhancement in the red emission. NIR-H2O2 is capable of fluorescent imaging of endogenously produced H₂O₂ in the living RAW264.7 macrophage cells. The mitochondria staining experiments suggest that the sensor mainly associates with the mitochondria of RAW264.7 macrophage cells.

In Vivo: The H₂O₂ production in vivo was generated by activated macrophages ａnd neutrophils in a lipopolysaccharide (LPS) model of acute inflammation. The mice treated with both LPS ａnd NIR-H2O2 exhibit a significantly higher fluorescence readout than the mice untreated or treated with only NIR-H2O2. The mice loaded with LPS ａnd NIR-H2O2 have approximately 10- ａnd 20-fold higher fluorescence intensity than the mice loaded with saline ａnd the sensor ａnd the mice loaded with saline, respectively.

相关产品：PEG300  |  SBE-β-CD  |  Corn oil  |  Tween 80  |  D-Lin-MC3-DMA  |  Polyethylene glycol 12-hydroxystearate  |  Lipopolysaccharides  |  JC-1  |  (2-Hydroxypropyl)-β-cyclodextrin  |  Methyl-β-cyclodextrin  |  Digitonin  |  3X FLAG peptide TFA  |  CFSE  |  DTT  |  Biotin  |  Tocofersolan  |  O-Propargyl-Puromycin  |  D-Luciferin potassium salt  |  Monocrotaline  |  Thiazolyl Blue  |  GsMTx4 TFA  |  Yoda 1  |  Nile Red  |  IPTG  |  FLAG peptide  |  Cremophor EL  |  5-Ph-IAA  |  Phosal 50 PG  |  Sodium carboxymethyl cellulose (Viscosity:800-1200 mPa.s)

品牌介绍:
•   MCE (MedChemExpress) 拥有数百种全球独家化合物，我们致力于为全球科研客户提供*新*全的高品质小分子活性化合物；
•   10,000 多种高选择性抑制剂、激动剂涉及各热门信号通路及疾病领域；
•   设有专业的实验中心和严格的质控、验证体系；
•   提供 LC/MS、NMR、HPLC、手性分析、元素分析等各项质检报告，确保产品的高纯度、高品质；
•   产品的生物活性多经各国客户实验验证；
•   Nature, Cell, Science 等多种期刊及制药收录了MCE客户的科研成果；
•   专业团队跟踪*新的制药及生命科学研究进展，为您提供全球*新的活性化合物；
•   与世界各大制药公司及知名科研机构建立了长期的合作。