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o-Pah

CAS No. : 1181844-41-6

MCE 站：o-Pah

产品活性：o-Pah 是一种带有 -NH2 和 -OH 取代的 meso-Ph 基团的 BODIPY 衍生物。o-Pah 在 Cu²⁺ 存在下，表现为金属诱导的 J 聚集和对 Hg²⁺ 特定荧光增强 (Ex/Em=483/(495-600) nM)。

研究领域：Others

作用靶点：Others

In Vitro: o-Pah (20 μM) shows changed colour upon addition of 100 μM different metal ions ａnd the further addition of 20 μM of Cu²⁺ in DMSO/HEPES buffer solution.
Procedures of metal ion sensing Stock solutions of the metal ions (100 mM ａnd 10 mM) were prepared in deionised water.
1. A stock solution of o-Pah (0.5 mM) is prepared in DMSO.2. The solution of o-Pah is then diluted to 5 μM with HEPES buffer solution (50 mM KNO3, 50 mM HEPRS, pH 7.2).3. During titration experiments, 2 mL solutions of o-Pah (5 μM, 0.5 μM) are placed in a 1 cm quartz optical cell ａnd Cu²⁺, Hg²⁺ stock solutions are added gradually with a micropipette.4. In selectivity experiments, the test samples are prepared by adding appropriate amounts of the metal ion stock solution into the 2 mL solution of o-Pah (5 μM). 6. During fluorescence measurements, the excitation wavelength is 483 nm ａnd emission spectra are collected between 495-600 nm.

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