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RNase 抑制剂

MCE 站：RNase 抑制剂

RNase Inhibitor 是在大肠杆菌表达系统中重组表达的 RNase 抑制剂，可特异性抑制 RNase A、B、C 三种酶的活性。

Description & Advantages:

RNase Inhibitor is a 50 kDa recombinant protein of murine origin expressed in E.coli. The inhibitor specifically inhibits RNase A, B ａnd C by binding noncovalently in a 1:1 ratio with high affinity. It is not effective against RNase 1, RNase T1, S1 Nuclease, RNase H or RNase from Aspergillus. In addition, no inhibition of polymerase activity is observed when RNase Inhibitor is used with Taq DNA Polymerase, AMV or M-MLV Reverse Transcriptases, or Phage RNA Polymerases (SP6, T7, or T3). RNase Inhibitor, Murine does not contain the pair of cysteines identified in the human version that is very sensitive to oxidation, so this product has significantly improved resistance to oxidation compared to the human/porcine RNase inhibitors, ａnd is stable at low DTT concentrations (less than 1 mM).

Application Features:

1.   cDNA synthesis

2.   RT-PCR

3.   In vitro transcription/translation

4.   Enzymatic RNA labeling reaction

5.   Other applications where the integrity of RNA is important

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