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SYBR Green I 核酸染料

MCE 站：SYBR Green I 核酸染料

SYBR Green I 是一种可代替溴化乙锭（EB）的新型花青类核酸染料，毒性很低，使用安全，可以检出 20 pg 的 dsDNA。

Description & Advantages:

SYBR Green I Nucleic Acid Gel Stain is a proprietary unsymmetrical cyanine dye that has proven exceptionally useful for assays requiring sensitive nucleic acid detection. SYBR Green I stain is maximally excited at 490 nm ａnd has secondary excitation peaks at 290 nm ａnd 380 nm. The fluorescence emission of SYBR Green I Stain bound to DNA is centered at 520 nm. The detection limit using SYBR Green I Stain is as low as 60 pg per band of dsDNA using 300 nm transillumination. With 254 nm epi-illumination, as little as 20 pg of dsDNA can be detected. SYBR Green I Stain is also possible to stain dsDNA prior to electrophoresis as it has an exceptionally high affinity for dsDNA.

   High sensitivity: Detect as little as 20 pg dsDNA per band

   High signal-to-noise ratio: strong fluorescence signal of the sample, low background signal

   Strong applicability: suitable for agarose gel or polyacrylamide gel electrophoresis; used for dsDNA, ssDNA or RNA staining

   No destaining or washing steps, compatible with different testing equipments

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