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Cyanine5.5 amine

CAS No. : 2097714-45-7

MCE 站：Cyanine5.5 amine

产品活性：Cyanine5.5 amine (Cy 5.5 amine)，Cy5.5 类似物，是一种近红外 (NIR) 荧光染料 (Ex=648 nm，Em=710 nm)。Cyanine5.5 amine 可用于制备 Cy5.5 标记的纳米颗粒，从而得以用共聚焦显微镜进行跟踪和成像，且荧光背景低。

研究领域：Others

作用靶点：Others

In Vivo: Real-time monitoring Cy5.5-labeled nanoparticles (Cy5.5-PLGA) in retinal blood vessels.
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, ａnd should be modified according to your specific needs).
1. Freshly prepared suspensions of Cy5.5-PLGA (resuspended in 0.5 ml 1% poloxamer 188, vortexed gently ａnd left for 30 min at ambient temperature before use) is administrated intravenously (0.5 mL).
2. Anesthetize the rats, treat the eyes with Neosynephrine-POS 5% to relax the iris, ａnd Vidisic eye gel is applied to protect the eye from drying out ａnd used as immersion medium for the contact lens as well.
3. Fix the rats under a confocal scanning microscope with the eye positioned in working distance underneath the objective lens, ａnd a cannula is inserted into the tail vein.
4. Observe the fluorescence in the retina, ａnd capture the images at different time points (0, 1, 3, 5, 15, 30, 60, 90 min).
Note: The rats are kept on the heating plate during all the in vivo imaging process.
5. After in vivo real-time imaging, rats were euthanized with an overdose of aforementioned anesthetic ａnd the eyeballs were enucleated ａnd placed into cooled HEPES buffered solution (135 mM NaCl, 5 mM NaOH, 2.5 mM KCl, 7 mM MgCl₂, 10 mM HEPES, 10 mM glucose; pH7.4).
6. Remove the anterior segment of eye ａnd vitreous body, separate whole retina carefully, flat on the modified culture plate.
7. Incubate the whole mounts with0.1 mg/mL Hoechst 33342 (HY-15559) in HEPES solution for 20 min for nuclei staining.
8. Fix flat mount retina with 4% paraformaldehyde solution for 20 min ａnd wash with HEPES solution.
9. Capture the images immediately with microscope after preparation of retinal flat mount.

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