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N-Aminofluorescein

CAS No. : 98907-26-7

MCE 站：N-Aminofluorescein

产品活性：N-Aminofluorescein 是一种螺旋形荧光素酰肼，是一种对 Cu²⁺ 具有高度选择性和敏感性的荧光探针。N-Aminofluorescein 对其他常见金属离子无选择性荧光响应，可用于生物体系中 Cu²⁺ 的直接检测 (λex/em=495/516 nm)。

研究领域：Others

作用靶点：Others

In Vitro: Guidelines (Following is our recommended protocol. This protocol only provides a guideline, ａnd should be modified according to your specific needs).
N-Aminofluorescein consists of a fluorescein moiety ａnd a hydrazide group to recognize ａnd bind Cu²⁺, can promote the hydrolysis of amide.
N-Aminofluorescein (FG) shows selectivity on Cu²⁺ ａnd shows the absorption ａnd emission bands at 632 nm ａnd 515 nm in 70% aqueous HEPES buffered solution (pH 7.4) containing Cu²⁺.
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, ａnd should be modified according to your specific needs).
General procedure for Cu²⁺ determination:
1.Prepare the 1.0 mM stock solution of spiro form fluorescein hydrazide in ethanol;
2.Conduct the fluorescence-on reaction in 0.01 M Tris-HCl buffer (pH 7.2), with 10 μM N-Aminofluorescein;
3.Add an appropriate volume of sample solution with a final Cu²⁺ concentration of not more than 10 μM, ａnd adjust the final volume 10 mL with 0.01 M Tris-HCl buffer (pH 7.2);
4.After 2 h, transfer a 3-mL portion of the solution to a 1-cm quartz cell, ａnd measure the fluorescence intensity/spectrum at room temperature with λex/em = 495/516 nm ａnd both excitation ａnd emission slit widths of 5 nm;
5.In the meantime, prepare a blank solution containing no Cu²⁺ ａnd measure with the same conditions for comparison.

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