产品中心

FG 488 DHPE

型号:

产品价格:电议      采购度:1195      原产地:美洲

发布时间:2022/9/28 19:23:13      所属地区:国外 国外

简要描述:

FG 488 DHPE 是一种脂质偶联荧光色素,已应用为荧光探针 Oregon Green 488。FG 488 DHPE 能够监测脂质囊泡的酸化 (λex/λem=508/534 nm)。FG 488 DHPE 也能用于 Hv1 诱导的质子转位定量 (λex/λem=508/534 nm)。

产品咨询 服务电话:
021-58955995
分享到:

标签:fg   

产品详情

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务。

FG 488 DHPE

CAS No. : 438476-80-3

MCE 站:FG 488 DHPE

产品活性:FG 488 DHPE 是一种脂质偶联荧光色素,已应用为荧光探针 Oregon Green 488。FG 488 DHPE 能够监测脂质囊泡的酸化 (λex/λem=508/534 nm)。FG 488 DHPE 也能用于 Hv1 诱导的质子转位定量 (λex/λem=508/534 nm)。

研究领域:Others

作用靶点:Others

In Vitro: FG 488 DHPE shows a pH-dependent fluorescence emission characteristic.

Monitoring acidification in Bulk vesicle assa:
1.Instrument: Jasco FP6500 spectrofluorometer, 37 ℃; fluorescence is excited at λex=508 nm and the emission is detected at λem=534 nm.
2.Add 100 L proteoliposomes (cphospholipid is about 60 M) to 680 L ATPase buffer, containing the K+-ionophore valinomycin (5 nM) to enable a charge equilibration for transported protons.
3.Add ATP (1.2 mM) to induce proton pumping.
4.Add 1 mM NaN3 to ATP hydrolysis.
5.Add CCCP (carbonyl cyanide 3-chlorophenyl hydrazine, 0.4 M) to deplete the proton gradient.
6.Conversion into pH-values, fluorescence intensities are normalized to the intensity obtained directly after ATP addition.

FG 488 DHPE exerts function in quantification of pH changes induced by the voltage-dependent proton channel Hv1.

Quantification of phospholipid concentrations:
1.Add Perchloric acid (70%, 200 μL) to a sample of unilamellar vesicles containing OG488-DHPE (30 μL).
2.Heat up to 220 °C for 60 min to generate inorganic phosphate.
3.Cooling down to room temperature, add 700 μL of a solution of NH4MoO4 (0.45% (w/v)) and perchloric acid (12.6% (w/v)) and 700 μL o f a 1.7% (w/v) acetic acid solution.
4.Obtain a calibration curve to know NaH2PO4 concentrations.
5.Incubated samples at 80 °C for 10 min and measure the absorption of the samples at 820 nm.
6.Calculate phospholipid concentrations of the vesicles using the calibration curve.

Proton translocation assay:
1.Instrument: Jasco FP6500 spectrofluorometer, 37 ℃; fluorescence is excited at λex=508 nm (3 nm band width) and the emission is detected at λem=534 nm (3 nm band width).
2.Dilute proteoliposomes composed of POPC/POPG/Chol/OG488-DHPE (54.5:25:20:0.5) in buffer A in flux buffer generating a 14-fold K+-gradient across the vesicular membrane.
3.Add valinomycin (13 nM) to cause protonation of OG488-DHPE and quench its fluorescence intensity in case of active Hv1 channels as described above.
4.Add CCCP (6 nM) to permeabilise all vesicles for protons.
5.The normalized fluorescence intensity Fnorm is plotted as a function of time. As a control for proton leakage, protein-free vesicles were used instead of proteoliposomes.
For the experiments in the presence of the potential inhibitor 2GBI, dissolve the inhibitor (15 mM) in flux buffer and add (0.5-8.0 μL) to the proteoliposomes before addition of valinomycin to induce proton translocation.

相关产品:Antitumor agent-67  |  MeOSuc-Ala-Ala-Pro-Val-pNA  |  BDP 558/568 amine  |  ATR-IN-9  |  TBTDC  |  (S)-Norfluoxetine-d5 (phenyl-d5)  |  H-4-Pal-OH  |  (1R,2S,7R)-Sitafloxacin-d4 hydrochloride  |  6-Hydroxy-4-methylcoumarin  |  Acridine Orange base  |  CEF19, Epstein-Barr Virus latent NA-3A (458-466)  |  AC-green  |  AMT-NHS  |  Nylidrin hydrochloride  |  Naphthalene-1,2-dicarbaldehyde  |  C13-112-tri-tail  |  (4S)-1-Boc-4-fluoro-D-proline  |  D-(+)-Fucose  |  OVA Peptide 323-339  |  Secretin (swine)  |  Bletilloside A  |  Transcriptional Intermediary Factor 2 (TIF2) (740-753)  |  RPR132595A-phenylmethyl hydrogen carbonate-d3  |  Fmoc-Ser(tBu)-OH-13C3,15N  |  Pep-1 (uncapped)  |  Aspochalasin M  |  Dihydrofuran-3(2H)-one  |  Rhodamine 800  |  (S)-Tamsulosin-d3 hydrochloride  |  BPK-29 hydrochloride

热门产品线:重组蛋白  |  化合物库  |  天然产物  |  染料试剂  |  PROTAC  |  同位素标记物  |  寡核苷酸

Trending products:Recombinant Proteins  |  Bioactive Screening Libraries  |  Natural Products  |  Dye Reagents  |  PROTAC  |  Isotope-Labeled Compounds  |  Oligonucleotides

品牌介绍:
•   MCE (MedChemExpress) 拥有数百种全球独家化合物,我们致力于为全球科研客户提供*新*全的高品质小分子活性化合物;
•   10,000 多种高选择性抑制剂、激动剂涉及各热门信号通路及疾病领域;
•   设有专业的实验中心和严格的质控、验证体系;
•   提供 LC/MS、NMR、HPLC、手性分析、元素分析等各项质检报告,确保产品的高纯度、高品质;
•   产品的生物活性多经各国客户实验验证;
•   Nature, Cell, Science 等多种期刊及制药收录了MCE客户的科研成果;
•   专业团队跟踪*新的制药及生命科学研究进展,为您提供全球*新的活性化合物;
•   与世界各大制药公司及知名科研机构建立了长期的合作。

1000+ Inhibitors&Agonists 作用于20多条经典信号通路
30+ Screening Libraries 疾病机制研究的高效工具
CCK8 Kit | Cell Counting Kit-8
FDA-Approved 药物筛选库
Inhibitor Cocktails 蛋白酶, 磷酸酶 & 去乙酰化酶
Top Publications Citing Use of MCE
MCE Hotline: 4008203792 | 中国现货 - 全球文献引用 - 高纯度高品质 - 全方位技术支持

更新时间:2024/1/2 10:26:30

留言咨询

  •  
  •  
  •  
  •  
  •  
  •  
  •  
验证码: 点击切换验证码

温馨提示

1.遵守中华人民共和国有关法律、法规,尊重网上道德,承担一切因您的行为而直接或间接引起的法律责任。
2.请您真实的反映产品的情况,不要捏造、诬蔑、造谣。如对产品有任何疑问,也可以留言咨询。
3.未经本站同意,任何人不得利用本留言簿发布个人或团体的具有广告性质的信息或类似言论。

相关新闻

相关产品

联系我们

电话:021-58955995
传真:021-53700325
邮箱:sales@medchemexpress.cn
地址:上海上海

版权所有©MedChemExpress, All Right Reseverd ICP备案号: 总访问量:10976654 管理登录 阿仪网 设计制作,未经允许翻录必究

8

阿仪网推荐收藏该企业网站

联系方式

18019480960
18019480960

工作时间

(24小时)