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Cyanine3 maleimide tetrafluoroborate

CAS No. : 2755154-93-7

MCE 站：Cyanine3 maleimide tetrafluoroborate

产品活性：Cyanine3 maleimide tetrafluoroborate 是一种巯基特异性标记试剂，λ_ex 为 550 nm，λ_em 为 580 nm。Cyanine3 maleimide tetrafluoroborate 可用于蛋白质和肽标记。

研究领域：Others

作用靶点：Others

In Vitro: Guidelines (Following is our recommended protocol. This protocol only provides a guideline, ａnd should be modified according to your specific needs).Labeling of Protein:1.The protein concentration was adjusted to 1 mg/mL for 100 μL.2. A 100 μL aliquot of the protein solution was mixed with Cyanine3 maleimide tetrafluoroborate (8 μg) in DMSO (2 μL).3. Incubated overnight at 4 °C in the dark.4. Labeled proteins were desalted using a G25 desalting column to remove excessive Cyanine3 maleimide tetrafluoroborate.5. Fluorescence intensities (FI) of labeled proteins were measured at excitation ａnd emission wavelengths of 550 nm ａnd 570 nm, respectively.MCE has not independently confirmed the accuracy of these methods. They are for reference only.

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