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Cy5 DBCO chloride

CAS No. : 2182601-72-3

MCE 站：Cy5 DBCO chloride

产品活性：Cy5 DBCO chloride 是一种叠氮化物反应探针，DBCO 分子的加入可以通过无铜 “Click Chemistry” 反应对叠氮化物标记的生物分子进行成像。

研究领域：Others

作用靶点：Others

In Vitro: Cy5 DBCO chloride (2-50 μM) can control the extent of labelling by varying the concentration of DBCO-Cy5 in a dose-dependent manner, with the amount of DBCO-Cy5 bound at the cell surface increasing with increasing DBCO-Cy5 concentration.
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, ａnd should be modified according to your specific needs).
1.Incubate the cells at a density of 3×10⁴ cells in a cell culture dish for 3 days ａnd then wash the cells twice with DPBS (pH 7.4). Incubate the cells according to your normal protocol.
2.Incubate the cells with Cy5 DBCO chloride at a final concentration of 20 M for 1 h at 37°C.
3.After incubation, wash cells with DPBS (pH 7.4) ａnd fix with formaldehyde-glutaraldehyde combination fixative for 15 min at room temperature.
4.After fixation, wash cells twice with DPBS (pH 7.4). Analyze sample on a flow cytometer, fluorescence microscopy, or fluorescence microplate reader.

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