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Flubida-2

CAS No. :

MCE 站：Flubida-2

产品活性：Flubida-2 是一种可渗透细胞的染料，可被细胞内的内酯酶水解为 Fubi-2 (水解后，Ex=492 nm, Em=517 nm)。通过将探针引导至亲和素融合蛋白所在的位置，Flubida-2 可用于检测细胞器中特定位点的 pH 值。

研究领域：Others

作用靶点：Others

In Vitro: Guidelines (Following is our recommended protocol. This protocol only provides a guideline, ａnd should be modified according to your specific needs).
1. Dissolve Flubida-2 in DMSO (approximately 2 mM).
2. Mix the stock solution 1: 1 with 20% (w/v in DMSO) Pluronic F-127 (Molecular Probes), ａnd dilute to the desired final concentration (2-4 μM) with serum-free DMEM.
3. 30 to 48 h post-transfection with either AV-KDEL or ST-AV DNA, HeLa cells are rinsed once with serum-free DMEM ａnd loaded with 2-4 μM Flubida-2 for 3-5 h (or overnight for 10-15 h).
4. Chase the labeled cells with normal growth medium for at least 2 h, to allow excess dye-biotin to exit from the cytosol.
5. The strong avidin-biotin interaction ensures stable, specific avidin-Flubi- binding that resisted washing. Biotin starvation of the cells is not necessary before Flubida- loading, as staining was bright ａnd stable.

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