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NBD-PE

CAS No. : 178119-00-1

MCE 站：NBD-PE

产品活性：NBD-PE 是一种有效的脂质荧光探针 (Excitation/Emission: 465/535 nm; Color: Green)。NBD-PE 在膜和细胞生物学方面有广泛的应用。

研究领域：Others

作用靶点：Others

In Vitro: Guidelines (Following is our recommended protocol. This protocol only provides a guideline, ａnd should be modified according to your specific needs).
Assay of phospholipid transfer activity:
1. The reaction mixture containes 2 mg mitochondrial protein, 0.02 μmol of NBD-PE, 0.5 mmol of Tris-HCl, pH 7.4, ａnd 0-200 μg of pH 5.l-supernatant protein.
2. Samples are incubated at 37°C for 1 hour with gentle agitation.
3. The reaction is terminated by placing samples in an ice bath for 10 min, followed by centrifugation at 15,000 g for 5 min.
4. The supernatants are decanted, allowed to come to room temperature, ａnd the absorbance (460 nm) ａnd the relative fluorescence at 535 nm (excitation at 465 nm) are determined.
5. Blanks containing only NBD-PE ａnd exchange protein are run concomitantly to correct for possible interaction between NBD-PE ａnd exchange protein.
6. The percent exchange of NBD-PE is calculated from the differences between the absorbances or relative fluorescence in the supernatant fractions in the presence ａnd absence of exchange protein.

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