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Rf470DL

CAS No. : 2758364-03-1

MCE 站：Rf470DL

产品活性：Rf470DL 是一种转子荧光 D-氨基酸 (RfDAA)。Rf470DL 可用于标记活细菌 (Ex=470 nm, Em=640 nm)。

研究领域：Anti-infection

作用靶点：Bacterial

In Vitro: Rf470DL can realize ash-free imaging of bacterial cell walls.
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, ａnd should be modified according to your specific needs).
1. RfDAA/FDAA stock solutions are prepared in DMSO at a concentration of 100 mM ａnd stored at -20°C before use.
2.1 For long-pulse labeling of B. subtilis ａnd E. coli, dilute the exponential phase cultures with fresh LB broth containing 1 mM Rf470DL to OD₆₀₀~0.05, ａnd incubate for 1 h.
2.2 Image immediately using a Nikon Ti-E inverted microscopy system without washing ａnd fixation.
3.1 For short-pulse labeling of S. venezuelae, Rf470DL stock solution is added directly to exponential phase cultures to a final concentration of 0.5 mM, followed by incubating at 30 °C with shaking for 15 min.
3.2 Image immediately using a Nikon Ti-E inverted microscopy system without washing ａnd fixation.

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