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Rose-β-D-Gal

CAS No. : 138182-21-5

MCE 站：Rose-β-D-Gal

产品活性：Rose-β-D-Gal 是荧光染料，也是 β-半乳糖苷酶底物。Rose-β-D-Gal 在反应后产生粉红色/品红的颜色，可用于检测 β-gal 活性。

研究领域：Others

作用靶点：Others

In Vitro: Guidelines (Following is our recommended protocol. This protocol only provides a guideline, ａnd should be modified according to your specific needs).Immunostaining of Tissues:
1.Prepare 25 mg/mL Rose-β-D-Gal stock solution in Dimethyl Sulfoxide (DMSO) ａnd store at -20 °C. It is vital to protect the stock solution from the light.
2.Prepare rinse buffer for Rose-β-D-Gal staining: 0.1 % Sodium Deoxycholate, 0.2 % IGEPAL CA-630, 2 mM MgCl₂ in 0.1 M Na Phosphate buffer (pH 7.3). Prepare 1,000 mL of 0.5 M Na Phosphate buffer (pH 7.3) by mixing with 158 mL of 1 M NaH₂PO₄, 342 mL of 1 M Na ₂HPO₄, ａnd 500 mL water.
3.Prepare substrate solution for Rose-β-D-Gal fresh, containing 1 mg/mL Rose-β-D-Gal, 5 mM Potassium Ferricyanide, ａnd 5 mM Potassium Ferrocyanide in the rinse buffer.
4.Rehydrate frozen tissue sections in PBS: quickly wash with PBS twice, wash with rinse buffer for 10 min 3 time.
5.Expose to β-galactosidase substrate Rose-β-D-Gal: stain at 37 ℃ for as long as needed to see stain, up to an overnight time period, keep covered ａnd in the dark during color development. Wash with PBS for 5 min twice.
6.Fix tissue in 4% PFA, unless on intend to continue with in situ hybridizations, ａnd continue to step 4.
Note: Rose-β-D-Gal is not very stable in alcohols or organic solvents, so must use water-based counterstains such as Gill’s hematoxylin ａnd cover slipping with aqueous mountant.

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