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MitoTracker Orange CMTMRos

CAS No. : 199116-50-2

MCE 站：MitoTracker Orange CMTMRos

产品活性：MitoTracker Orange CMTMRos 是一种荧光染料，利用线粒体膜电位标记活细胞内的线粒体 (Ex/Em: 551/576 nm)。

研究领域：Others

作用靶点：Others

In Vitro: Guidelines (Following is our recommended protocol. This protocol only provides a guideline, ａnd should be modified according to your specific needs).
Labeling of Cells:
For live cells, follow Steps 17. For fixed cells, follow Steps 813.
1. Prepare a labeling solution at 25500 nM in the desired cell culture medium, ａnd warm it to the cell culture temperature.
2. Aspirate the culture medium from cells grown on coverslips.
3. Immerse the cells in the labeling medium for 1545 min at 37C.
4. Aspirate the labeling medium, ａnd rinse the cells three times with culture medium.
5. Mount the cells.
6. Check the cells using a fluorescence microscope. If the fluorescence staining is too low, try one of the following options:
i. Incubate for an additional 30 min in the normal culture medium to allow the thiol conjugation to proceed.
ii. Try a higher initial concentration of the labeling solution.
7. When the fluorescence is sufficient, image the cells.
8. Carry out Steps 14, but use a labeling solution at a working concentration between 100 ａnd 1000 nM.
9. Rinse the cells three times with PBS+.
10. Fix the cells for 10 min in 3.7% formaldehyde in PBS (pH 7.4).
11. Aspirate ａnd rinse the cells three times for 5 min each in PBS.
12. Mount the coverslips.
13. Image the cells.

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