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Mag-indo-1/AM

CAS No. : 130926-94-2

MCE 站：Mag-indo-1/AM

产品活性：Mag-indo-1/AM 是一种具有细胞渗透性的镁离子荧光指示剂，也可指示钙离子 (Ex=340-390 nm, Em=410-490 nm)。Mag-indo-1/AM 可用于测定细胞内镁离子和钙离子浓度。

研究领域：Others

作用靶点：Others

In Vitro: Guidelines (Following is our recommended protocol. This protocol only provides a guideline, ａnd should be modified according to your specific needs).
Measurement of intracellular Ca²⁺:
1. Incubate 3 mL cell suspension with 10 M Mag-indo-1/AM for 15 min at 30℃.
2. Centrifuge the cells for 1 min at 500 rpm ａnd resuspend in 5 mL isolation buffer (containing 1 mM CaCl₂).
3. Add 1 mM probenecid to the cell suspension to prevent dye leakage from the cytosol ａnd store the cells (Mag-indo-1/AM-loaded cells) at room temperature until use.
4. Incubate the Mag-indo-1/AM-loaded cells at room temperature for 2 h, shake gently at 37℃ for 1.5 h, centrifuge the pellet, resuspend ａnd store at room temperature.
6. Place a small portion of the loaded cells in an experimental chamber which is mounted on the stage of an inverted microscope.
7. Measure fluorescence of Mag-indo-1/AM (Ex=340-390 nm, Em=410 ａnd 490 nm (the peak emissions of the Ca²⁺ bound ａnd Ca²⁺ free forms of the indicator)). Fluorescence of the whole cell is collected on line by a photon counting system.

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