MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务。

C6 NBD Ceramide

CAS No. : 94885-02-6

MCE 站：C6 NBD Ceramide

产品活性：C6 NBD Ceramide 是一种具有细胞膜渗透性的高尔基体荧光探针 (Ex=466 nm, Em=536 nm)。C6 NBD Ceramide 能快速便捷地在活体细胞和固定细胞中对高尔基体进行绿色荧光标记，可用于观察活细胞中高尔基体形态的变化。C6 NBD Ceramide 能被代谢为荧光鞘磷脂和葡糖基甘油酰胺，可用于鞘脂转运和代谢机制的研究。

研究领域：Others

作用靶点：Others

In Vitro: Guidelines (Following is our recommended protocol. This protocol only provides a guideline, ａnd should be modified according to your specific needs).
Labeling of Golgi apparatus:
1. Rinse cells (on glass cover slips) by HMEM, then fix cells in glutaraldehyde or paraformaldehyde for 5-10 min (25℃).
2. Wash fixed cells in a balanced salt solution (such as HCMF).
3. Transfer culture dishes containing the glass cover slips to an ice-water bath ａnd incubate with freshly prepared NaBH₄ for 3×5 min (for glutaraldehyde-fixed cells).
4. Rinse cells several times (20-30 min) in cold HCMF, warm to 25℃, then incubate with C6 NBD-ceramide/BSA for 60 min.
5. Wash fixed cells by HCMF, ａnd incubate with 10% fetal calf serum or 2 mg/mL BSA (30-90 min; 25℃) to back-exchange excess C6 NBD-ceramide from the preparation ａnd enhance the staining of the Golgi apparatus.
6. The glass cover slips mounted on depression slides ａnd observe by fluorescence microscope (Ex=466 nm, Em=536 nm).

相关产品：Antitumor agent-67  |  MeOSuc-Ala-Ala-Pro-Val-pNA  |  BDP 558/568 amine  |  ATR-IN-9  |  TBTDC  |  (S)-Norfluoxetine-d5 (phenyl-d5)  |  H-4-Pal-OH  |  (1R,2S,7R)-Sitafloxacin-d4 hydrochloride  |  6-Hydroxy-4-methylcoumarin  |  Acridine Orange base  |  CEF19, Epstein-Barr Virus latent NA-3A (458-466)  |  AC-green  |  AMT-NHS  |  Nylidrin hydrochloride  |  Naphthalene-1,2-dicarbaldehyde  |  C13-112-tri-tail  |  (4S)-1-Boc-4-fluoro-D-proline  |  D-(+)-Fucose  |  OVA Peptide 323-339  |  Secretin (swine)  |  Bletilloside A  |  Transcriptional Intermediary Factor 2 (TIF2) (740-753)  |  RPR132595A-phenylmethyl hydrogen carbonate-d3  |  Fmoc-Ser(tBu)-OH-13C3,15N  |  Pep-1 (uncapped)  |  Aspochalasin M  |  Dihydrofuran-3(2H)-one  |  Rhodamine 800  |  (S)-Tamsulosin-d3 hydrochloride  |  BPK-29 hydrochloride

热门产品线：重组蛋白  |  化合物库  |  天然产物  |  染料试剂  |  PROTAC  |  同位素标记物  |  寡核苷酸

Trending products：Recombinant Proteins  |  Bioactive Screening Libraries  |  Natural Products  |  Dye Reagents  |  PROTAC  |  Isotope-Labeled Compounds  |  Oligonucleotides

品牌介绍:
•   MCE (MedChemExpress) 拥有数百种全球独家化合物，我们致力于为全球科研客户提供*新*全的高品质小分子活性化合物；
•   10,000 多种高选择性抑制剂、激动剂涉及各热门信号通路及疾病领域；
•   设有专业的实验中心和严格的质控、验证体系；
•   提供 LC/MS、NMR、HPLC、手性分析、元素分析等各项质检报告，确保产品的高纯度、高品质；
•   产品的生物活性多经各国客户实验验证；
•   Nature, Cell, Science 等多种期刊及制药收录了MCE客户的科研成果；
•   专业团队跟踪*新的制药及生命科学研究进展，为您提供全球*新的活性化合物；
•   与世界各大制药公司及知名科研机构建立了长期的合作。