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Gol-NTR

CAS No. :

MCE 站：Gol-NTR

产品活性：Gol-NTR 是一种高选择性和高灵敏度的高尔基体靶向探针。Gol-NTR 是硝基还原酶 (NTR) 激活的，具有可视化急性肺损伤 (ALI) 和修复功能。Gol-NTR 可用于监测和评估脓毒症诱导的 ALI 治疗反应的研究。

研究领域：Others

作用靶点：Others

In Vivo: Guidelines (Following is our recommended protocol. This protocol only provides a guideline, ａnd should be modified according to your specific needs) .
In Vivo Imaging:
1. C57BL/6 mice (aged 6-8 weeks, 20-22 g, male) are purchased. After acclimating for 1 week, mice are randomly assigned to different groups.
2. All animal operations are performed in accordance with the guidelines for animal experimentation.
3. All of the mice are sacrificed by cervical vertebra dislocation, ａnd collected immediately lung organs.
4. After thoroughly washing with PBS, the lungs are incubated with Gol-NTR (50 μM) in PBS for 1 h.
5. The organs are washed with PBS to remove excess Gol-NTR ａnd then subjected to fluorescence imaging on a small animal imaging system with an excitation filter of 420 nm ａnd an emission filter of 510 nm.

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