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Cy3 methyltetrazine

CAS No. : 2183473-57-4

MCE 站：Cy3 methyltetrazine

产品活性：Cy3 methyltetrazine (TZ-Cy3) 是一种具有甲基四嗪结构单元的点击化学试剂，其对环辛烯具有高度反应性。Cy3 methyltetrazine 也是一种四嗪修饰的荧光探针，可用于分析溶液和活细胞中的蛋白质磷酸化。

研究领域：Others

作用靶点：Others

In Vitro: Guidelines (Following is our recommended protocol. This protocol only provides a guideline, ａnd should be modified according to your specific needs).
Confocal imaging of phosphorylation in cells:
1. Culture H1299 cells in a coverglass-bottom imaging dish for 24 h (take H1299 cells as an example).
2. Incubate H1299 cells with different concentrations of ATP-NB (a ATP analogue functionalized by norbornene, which can penetrate cells ａnd efficiently phosphorylate proteins in living cells) for 1 h.
3. Add Cy3 methyltetrazine to label the phosphorylated protein with fluorescence.
4. Incubate with fresh medium for 1 h.
5. Wash the cells twice with PBS to release the free Cy3 methyltetrazine probe.
6.Incubate the cells with 4% paraformaldehyde for 15 min ａnd with 0.1% Triton X-100 solution at room temperature for 10 min.
7. Wash cells 4 times with PBS.
8. Use a confocal microscopy imaging system to obtain the high-resolution images of cells (Ex=488 nm; Em=561 nm).

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