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WSP-1

CAS No. : 1352750-34-5

MCE 站：WSP-1

产品活性：WSP-1 是一种选择性的、快速反应的 H₂S 特异的荧光染料 (Ex/Em=465/515 nm)。WSP-1 与 H₂S 反应并释放荧光团。

研究领域：Others

作用靶点：Fluorescent Dye

In Vitro: WSP-1 have fluorescence properties, with excitation wavelength=465 nm, emission spectrum=515 nm.
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, ａnd should be modified according to your specific needs).
Fluorimetric Detection of Intracellular H₂S (Fluorimetric Microscope Assessment):
1. Culture HASMCs up to about 90% confluence, ａnd 24 h before the experiment, seed cells onto a 8-well culture slide at density of 30*10³ per well ａnd culture for 24 hours.
2. Replace the culture medium, ａnd incubate the cells for 30 min at 37°C in 400 μL per well WSP-1 working solution for 30 min.
3. Replace the WSP-1 working solution with 380 μL of buffer standard ａnd add 20 μL of the tested H2S-donor compounds at the desired concentration.
4. When WSP-1 reacts with H₂S, it releases a fluorophore detect able at Ex/Em=465/515 nm. Remove the buffer standard, ａnd wash with 400 μL of DPBS after 1 h incubation.
5. Remove the DPBS, ａnd add 200 μL of Bouin solution per well for 10 min at room temperature to fix the cells. Remove the excess of Bouin solution ａnd wash the cells twice with 400 μL of DPBS.
6. Acquire the fluorescence signal with a fluorescence microscope.

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