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IL-8/CXCL8 Protein, Human (72a.a, HEK293, His)

MCE 站：IL-8/CXCL8 Protein, Human (72a.a, HEK293, His)

白细胞介素8 (IL-8)，也称为 CXCL8 或 NAP-1，是一种促炎 CXC 趋化因子。IL-8 通过两种受体 CXCR1 和 CXCR2 作用于人类中性粒细胞。IL-8 具有保守的Glu-Leu-Arg (ELR) N 端基序，是CXCR1/CXCR2 的激动剂。IL-8 由各种细胞产生，包括白细胞、内皮细胞和上皮细胞。IL-8/CXCL8 Protein, Human (77a.a, HEK293) 是在 HEK293 细胞中产生的，由 77 个氨基酸(A23-S99) 组成。

Synonyms: C-X-C motif chemokine 8; MDNCF; Emoctakin; NAP-1

Species: Human

Source: HEK293

Biological Activity: Immobilized Human IL-8, His Tag at 0.5 μg/mL (100μl/well) on the plate. Dose response curve for Anti-IL-8 Antibody, hFc Tag with the EC₅₀ of <13.6 ng/mL determined by ELISA.

研究背景：IL-8 (CXCL8) belongs to the ELR⁺ CXC chemokines family. IL-8 is initially produced as a protein of 99 amino acids that undergoes cleavage to form active IL-8 isoforms, a 77 amino acid peptide in non-immune cells or a 72 amino acid peptide in monocytes ａnd macrophages. The gene encoding IL-8 is located on chromosome 4q13-q21. Dimerisation of IL-8 forms the structural basis for receptor binding. IL-8 is expressed by various cells including monocytes, macrophages, leukocytes, endothelial cells, ａnd epithelial cells.
Mature human IL-8/CXCL8 shares 75% amino acid sequence identity with canine IL-8/CXCL8. While, human IL-8 shares 94.95% aa sequence identity with Rhesus Macaque IL-8 protein.
IL-8 is responsible for the recruitment ａnd activation of neutrophils ａnd granulocytes to the site of inflammation. IL-8 is almost undetectable in physiological states, but is rapidly induced by pro-inflammatory cytokines such as TNFα ａnd IL-1β. The function of IL-8 mainly relies on its interaction with specific cell surface GPCR, CXCR1 ａnd CXCR2. In addition, IL-8 is reported to promote integrin β3 upregulation ａnd the invasion of hepatocellular carcinoma cells through activation of the PI3K/Akt pathway. In odontogenic lesions, IL-8 has been proven to be highly expressed in ameloblastoma epithelial cells ａnd irreversible pulpitis. In rheumatoid arthritis ａnd other inflammatory joint diseases IL-8 could bring about the accumulation of neutrophils, which are considered a major source of cartilage-degrading enzymes. IL-8 stimulates the MAPK ａnd tyrosine phosphorylation of cellular proteins. Tumour cells ａnd fibroblasts communicate with each other, including autocrine ａnd paracrine factors, including IL-8, resulting in the upregulation of MMP2 ａnd MMP9 degradable extracellular matrix (ECM) components that trigger tumour invasion.
IL-8 is typically known to promote angiogenesis, but it also activates matrix metalloproteinase (MMP) that is involved in metastasisrelated tissue remodelling. IL-8 is induced in lipopolysaccharide (LPS)-stimulated monocytes ａnd shown to induce neutrophil migration. IL-8 exerts multiple effects on biological activities of tumour cells including proliferation, invasion ａnd migration. IL-8 also increases the expression of Akt in androgen-independent prostate cancer (AIPC) cell lines. IL-8 activates MAPK signalling via PI3K in neutrophils, ａnd via transactivation of EGFR resulting in Ras-GTPase activation in ovarian ａnd lung cancer cell lines. There is substantial amount of experimental data suggesting that IL-8 ａnd receptors contribute to elimination of pathogens, but may also contribute significantly to disease-associated processes, including tissue injury, fibrosis, angiogenesis ａnd tumorigenesis.

蛋白编号：P10145 (S28-S99)

基因 ID：3576

产品分类：细胞因子和生长因子  |  趋化因子  |  CXC 趋化因子  |  CXC 趋化因子配体 8

Categories：Cytokines ａnd Growth Factors  |  Chemokine & Receptors  |  CXC Chemokines  |  IL-8/CXCL8

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