MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务。

Collagenase, Type III

CAS No. : 9001-12-1

MCE 站：Collagenase, Type III

产品活性：Collagenase, Type III 是一种来源于微生物的基质金属蛋白酶 (MMP) 和锌肽酶。Collagenase, Type III 分解胶原蛋白1、4、9、10、14、纤连蛋白、MMP-9、明胶、纤溶酶原、聚集蛋白聚糖、基底膜骨连接蛋白。

研究领域：Metabolic Enzyme/Protease

作用靶点：MMP

In Vitro: Type III collagenase is recommended for breast primary cell isolation.

Preparation of storage solution
1. Add 1 mL Hank＇s Balanced Salt Solution (HBSS) with calcium ａnd magnesium directly to 1 g vial of Collagenase.Vortex gently to ensure complete dissolution, ａnd prepare a stock solution of 100 mg/mL (100X stock solution).
2. Filter sterilize 100X stock solution using a 0.22 μm filter with a low protein binding filtration unit. Use immediately or dispense into aliquots ａnd store at -20°C to -5°C protected from light.
3. Thaw on ice prior to use. Commonly used concentrations for tissue ａnd cell dispersion are 0.5-2.5 mg/mL ａnd for cartilage digestion are 1-2 mg/mL, but the optimal working concentration required needs to be determined based on specific experimental conditions or by referring to the appropriate literature.
Dissociate Tissue
1. Mince tissue into 3-4 mm pieces with a sterile scalpel or scissors.
2. Wash the tissue pieces several times with HBSS containing calcium ａnd magnesium.
3. Add sufficient HBSS with calcium ａnd magnesium to submerge tissue. Add collagenase to required working concentration.
4. Incubate at 37°C for 4-18 hours. Increased efficiency is obtained using a rocker platform ａnd supplementing the digest with 3 mM CaCl₂.
5. Disperse cells by passing through a sterile stainless steel or nylon mesh. Remaining tissue fragments may be disaggregated by addition to fresh collagenase solution ａnd further incubation at 37°C.
6. Wash dispersed cells several times by centrifugation in HBSS w/o collagenase.
7. Resuspend cell pellet, after the final wash step, in culture medium. Determine viable cell density using a Automated Cell Counter (alternate automated or manual methods may be used).
8. Seed cells into culture vessels containing appropriate media.
Organ Perfusion
1. Add collagenase to prewarmed (37°C) HBSS with calcium ａnd magnesium. Addition of 3 mM CaCl₂ increases the efficiency of dissociation.
2. Perfuse organ at preoptimized rate for the particular organ.
3. Dispersed cells ａnd tissue fragments are separated from larger pieces by passing the perfusate through a sterile stainless steel or nylon mesh. Remaining tissue fragments may be disaggregated by addition to fresh collagenase solution ａnd further incubation at 37°C.
4. The steps are the same as for tissue isolation 6-8.

相关产品：Doxycycline hyclate  |  T-5224  |  Ilomastat  |  Chloramphenicol  |  Astragaloside IV  |  Edaravone  |  Isoginkgetin  |  Cordycepin  |  Marimastat  |  GI254023X  |  Stigmasterol  |  Salvianolic acid A  |  o-Phenanthroline  |  Actinonin  |  Meloxicam  |  Arctigenin  |  Batimastat  |  SB-3CT  |  GW280264X  |  MMP-9-IN-1  |  Chondroitin sulfate  |  Aderbasib  |  TAPI-1  |  (-)-Epigallocatechin  |  TAPI-2  |  Tranexamic acid  |  Incyclinide  |  Indinavir sulfate

Trending products：Recombinant Proteins  |  Bioactive Screening Libraries  |  Natural Products  |  Dye Reagents  |  PROTAC  |  Isotope-Labeled Compounds

品牌介绍:
•   MCE (MedChemExpress) 拥有200 多种全球独家化合物库，我们致力于为全球科研客户提供前沿*全的高品质小分子活性化合物；
•   50,000 多种高选择性抑制剂、激动剂涉及各热门信号通路及疾病领域；
•   产品种类涵盖各种重组蛋白，多肽，常用试剂盒 ，更有 PROTAC、ADC 等特色产品，广泛应用于新药研发、生命科学等科研项目；
•   提供虚拟筛选，离子通道筛选，代谢组学分析检测分析，药物筛选等专业技术服务；
•   设有专业的实验中心和严格的质控、验证体系；
•   提供 LC/MS、NMR、HPLC、手性分析、元素分析等各项质检报告，确保产品的高纯度、高品质；
•   产品的生物活性多经各国客户实验验证；
•   Nature, Cell, Science 等多种期刊及制药收录了MCE客户的科研成果；
•   专业团队跟踪*新的制药及生命科学研究进展，为您提供全球*新的活性化合物；
•   与世界各大制药公司及知名科研机构建立了长期的合作。