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Photo-lysine hydrochloride

CAS No. :

MCE 站：Photo-lysine hydrochloride

产品活性：Photo-lysine hydrochloride，一种新的基于赖氨酸光反应性的氨基酸，它捕获结合赖氨酸翻译后修饰的蛋白质。

研究领域：Others

作用靶点：Others

In Vitro: Photo-lysine is designed ａnd synthesized by incorporating a photo-cross-linker (diazirine) into the side chain of natural lysine. Photo-lysine, which is readily incorporated into proteins by native mammalian translation machinery, can be used to capture ａnd identify proteins that recognize lysine post-translational modifications (PTMs), including ‘readers’ ａnd ‘erasers’ of histone modifications. Photo-lysine can be incorporated into MDH2 ａnd mediate photo-cross-linking to fix protein-protein interactions in cells. UV irradiation of cells in the presence of photo-lysine induced robust cross-linking of HSP90β ａnd HSP60. Photo-lysine has higher efficiency than photo-leucine for photo-cross-linking of the two chaperone proteins. Photo-lysine enables capture of the heterodimer of proteins Ku70 ａnd Ku80 within a protein complex. Photo-lysine enables identification of histone- ａnd chromatin-binding proteins.

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